WE STUDY MECHANISMS OF B CELL AND ANTIBODY IMMUNODOMINANCE DURING ANTI-VIRAL RESPONSES
We are particularly interested in defining the key forces driving immunodominance following influenza virus infection and vaccination. We use a combination of flow cytometry, microscopy, immunological assays, antibody and single-cell sequencing methods. Our ultimate goal is to apply this knowledge in order to Improve current and develop new vaccines.
Determinants of Immunodominance
Immunodominace is the phenomenon of unequal immunogenicity between different antigens in a complex mixture or between different antigenic sites on the same antigen. We are exploring how variation of individual factors alters immunodominance in response to viral infection.
Contribution of Pre-existing Immunity
Pre-existing immunity influence subsequent immune responses. Humans are continuously exposed to influenza virus infections and vaccinations. We are currently working on understanding how pre-existing memory B cells, antibodies and CD4 T cells affect secondary immune response to the same antigen.
B cell Transcriptional Signatures
There is currently little or no information linking B cell receptor (BCR) sequence, mutational load and total transcriptome to antigenic site specificity. We are studying B cells, at different developmental stages, specific for distinct antigenic sites in various organis in order to link transcriptional programming with epitope specificity.